introduction: several potyviruses infecting gramineous plants have been reported from iran. mosaic is the most widespread viral disease affecting sugarcane and maize production. sugarcane mosaic virus (scmv) is one of the causal agents of mosaic disease, which was first described in ohio in 1963. scmv belonging to the genus potyvirus, family potyviridae, is a single-stranded positive sense rna ...

Sugarcane mosaic disease is caused by the Sugarcane streak mosaic virus (SCSMV; genus Poacevirus, family Potyviridae) which is common in some Asian countries. Here, we established a protocol of a one-step real-time quantitative reverse transcription PCR (real-time qRT-PCR) using the TaqMan probe for the detection of SCSMV in sugarcane. Primers and probes were designed within the conserved regio...

Sugarcane mosaic caused by Sugarcane Mosaic Virus (SCMV) is one of the most important virus diseases of sugarcane. In the present study, changes in the transcription profile obtained by cDNA-AFLP analysis were investigated in two sugarcane varieties contrasting to SCMV resistance, when challenged with a severe virus strain. Healthy plants derived from meristem tip tissue culture were mechanical...

A sensitive duplex-immunocapture-RT-PCR (D-IC-RT-PCR) technique was developed for detection and discrimination of taxonomically distinct Sugarcane streak mosaic virus (SCSMV) and Sugarcane mosaic virus (SCMV) that naturally infect sugarcane. D-IC-RT-PCR was performed using polyclonal antisera for capture of virions. Oligo 5'-d(T)18(AGC)-3' as a common reverse primer for both viruses and virus s...

Sugarcane mosaic potyvirus (SCMV) UP-isolate was antigenically similar to SCMV-A, SCMVMDB and two SCMV isolates from Thailand. The results were confirmed either by DAC-ELISA, EBIA, DIBA and ISEM independently or in combination. No reaction was evident with antiserum specific for other members of the Sugarcane mosaic potyvirus subgroup or any other potyviruses tested. The peptide profiling of a ...

A variant ofSCMV-D is the most common strain of sugarcane mosaic virus (SCMV) occurring in sugarcane in the coastal hinterland and high altitude areas of Natal. In field trials conducted at Mount Edgecombe and Eston this strain decreased the yield of recoverable sugar from variety NCo 376 by 30 to 42 % and that from variety Nl2 by 15 to 30% when all the stalks were infected. Losses in yield wer...

Among seed-transmitted cereal viruses the most important is Barley stripe mosaic virus (BSMV), infecting barley. Investigations on the occurrence of BSMV in Poland have been carried out in the Institute of Plant Protection in Poznań since 2000. The studies aimed at the evaluation of the BSMV distribution in plant material, the rate of its seed transmission in different barley cultivars and the ...